Group of Prof. Tinnefeld - Faculty for Chemistry and Pharmacy




J. Vogelsang, T. Cordes, P. Tinnefeld (2009): Single-molecule photophysics of oxazines on DNA and its application in a FRET switch, Photochem. Photobiol. Sci. 8, 486-496.

T. Cordes, I.H. Stein, C. Forthmann, C. Steinhauer, M. Walz, W. Summerer, B. Person, J. Vogelsang, P. Tinnefeld (2009): Controlling the emission of organic dyes for high sensitivity and superresolution microscopy, Proc SPIE 7367, 73671D.


C. Steinhauer, R.Jungmann, T.L. Sobey, F.C. Simmel, P. Tinnefeld (2009): DNA origami as a nanoscopic ruler for super-resolution microscopy, Angew. Chem. Int. Ed. 48(47), 8797 - 8999.


B. Person, I.H. Stein, C. Steinhauer, J. Vogelsang, P. Tinnefeld (2009): Correlated movement and bending of nucleotide acid structures visualized by multicolor single-molecule spectroscopy, ChemPhysChem 10(9-10), 1455-1460.


T. Cordes, J. Vogelsang, P. Tinnefeld (2009): On the mechanism of Trolox as antiblinking and antibleaching reagent, J. Am. Chem. Soc. 131(14), 5018-5019.


J. Vogelsang, T. Cordes, C. Forthmann, C. Steinhauer, P. Tinnefeld (2009): Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy, Proc. Natl. Acad. Sci. USA 106(20), 8107-8112.



M. Heilemann, S. van de Linde, M. Schuttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, M. Sauer (2008): Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes, Angew. Chem. Int. Ed. 47(33), 6172-6176.

J. Vogelsang, R. Kasper, C. Steinhauer, B. Person, M. Heilemann, M. Sauer, P. Tinnefeld (2008): A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes, Angew. Chem. Int. Ed. 47(29), 5465-5469

including cover picture


S. Huang, Q. X., Z.K. He, Y. Liu, P. Tinnefeld, X.R. Su, X.N. Peng (2008): A High Sensitive and Specific QDs FRET Bioprobe for MNase, Chem. Comm. 45, 5990-5992.


C. Steinhauer, C. Forthmann, J. Vogelsang, P. Tinnefeld (2008): Superresolution Microscopy on the Basis of Engineered Dark States, J. Am. Chem. Soc. 130(50), 16840–16841.


B. Seefeldt, R. Kasper, T. Seidel, P. Tinnefeld, K.-J. Dietz, M. Heilemann, M. Sauer (2008): Fluorescent proteins for single-molecule fluorescence applications, J. Biophoton. 1, 74-82.



P. Tinnefeld, J. Mattay, K.-H. Drexhage, M. Sauer (2007): Optical switches: Key molecules for improved fluorescence imaging and tracking with high optical resolution, Proc SPIE 6633, 663303.

J. Vogelsang, S. Doose, M. Sauer, P. Tinnefeld (2007): Improving single-molecule FRET measurements by confining molecules in nanopipettes, Proc SPIE 6633, 66331L.


P. Tinnefeld (2007): Making ultrasensitive weighing biocompatible by placing the sample within a resonant cantilever, Angew. Chem. Int. Ed. 46(42), 7926-7929.


J. Vogelsang, S. Doose, M. Sauer, P. Tinnefeld (2007): Single-molecule fluorescence resonance energy transfer in nanopipets: improving distance resolution and concentration range, Anal. Chem. 79(19), 7367-7375.


J.N. Clifford, T.D.M. Bell, P. Tinnefeld, M. Heilemann, S.M. Melnikov, J.-i. Hotta, M. Sliwa, P. Dedecker, M. Sauer, J. Hofkens. E.K.L. Yeow (2007): Fluorescence of single molecules in polymer films: sensitivity of blinking to local environment, J. Phys. Chem. B 111(25), 6987-91.


J. Roß, P. Buschkamp, D. Fetting, A. Donnermeyer, C.M. Roth, P. Tinnefeld (2007): Multicolor Single-Molecule Spectroscopy with Alternating Laser Excitation for the Investigation of Interactions and Dynamics, J. Phys. Chem. B 111(2), 321-326

including cover picture


P. Tinnefeld, M. Sauer (2007): Molecular optical switches and waveguides, Optik&Photonik 2, 45-48.


R. Kasper, M. Heilemann, P. Tinnefeld, M. Sauer (2007): Towards ultra-stable fluorescent dyes for single-molecule spectroscopy, Proc SPIE 6633, 66331Z.


S. Henkenjohann, S. van de Linde, S. Doose, P. Tinnefeld, M. Sauer (2007): Photoinduced electron transfer (PET)-probes for the study of enzyme activity at the ensemble and single molecule level, Proc SPIE 6633, 663323.


D. Fetting, R. Kasper, P. Tinnefeld (2007): Multicolor single-molecule spectroscopy for the study of complex interactions and dynamics, Proc SPIE 6633, 66330H.



G. Sánchez-Mosteiro, E.M.H.P. van Dijk, J. Hernando, M. Heilemann, P. Tinnefeld, M. Sauer, F. Koberling, M. Patting, M. Wahl, R. Erdmann, N.F. van Hulst, M.F. Garcia-Parajó (2006): DNA-based molecular wires: multiple emission pathways of individual constructs, J. Phys. Chem. B 110(51), 26349-26353.

M. Heilemann, R. Kasper, P. Tinnefeld, M. Sauer (2006):Dissecting and reducing the heterogeneity of excited-state energy transport in DNA-based photonic wires, J. Am. Chem. Soc. 128(51), 16864-16875.


P. Tinnefeld (2006): Detection of single oxygen molecules opens up new vistas for the investigation of molecular cooperativity in hemocyanins, ChemPhysChem 7(6), 1189-1191.


R. Kurtz, M. Fricke, J. Kalb, P. Tinnefeld, M. Sauer (2006): Application of multiline two-photon microscopy to functional in vivo imaging, J. Neurosci. Methods 151(2), 276-286.


E. Margeat, A.N. Kapanidis, P. Tinnefeld, Y. Wang, J. Mukhopadhyay, R.H. Ebright, S. Weiss (2006): Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes, Biophys. J. 90(4), 1419-1431.


A. Biebricher, M. Sauer, P. Tinnefeld (2006): Radiative and non-radiative rate fluctuations of single semiconductor nanocrystals, J. Phys. Chem B 110(11), 5174-5178.



T. Heinlein, A.s Biebricher, P. Schluter, C.M. Roth, D.-P. Herten, J. Wolfrum, M. Heilemann, C. Müller, P. Tinnefeld, M. Sauer (2005): High-resolution colocalization of single molecules within the resolution gap of far-field microscopy, ChemPhysChem 6(5), 949-955.

P. Tinnefeld, M. Sauer (2005): Branching out of single-molecule fluorescence spectroscopy: challenges for chemistry and influence on biology, Angew. Chem. Int. Ed. 44(18), 2642-2671.


M. Heilemann, E. Margeat, R. Kasper, M. Sauer, P. Tinnefeld (2005): Carbocyanine dyes as efficient reversible single-molecule optical switch. - J Am Chem Soc, 127(11), 3801-3806.


P. Tinnefeld, M. Heilemann, M. Sauer (2005): Design of molecular photonic wires based on multistep electronic excitation transfer, ChemPhysChem 6(2), 217-222.



P. Tinnefeld, J. Hofkens, D.-P. Herten, S. Masuo, T. Vosch, M. Cotlet, S. Habuchi, K. Müllen, F.C. De Schryver, M. Sauer (2004): Higher-excited-state photophysical pathways in multichromophoric systems revealed by single-molecule fluorescence spectroscopy, ChemPhysChem 5(11), 1786-1790.

A. Biebricher, A. Paul, P. Tinnefeld, A. Gölzhäuser, M. Sauer (2004): Controlled three-dimensional immobilization of biomolecules on chemically patterned surfaces, J. Biotechnol. 112(1-2), 97-107.


M. Heilemann, P. Tinnefeld, G. Sánchez Mosteiro, M. Garcia Parajo, N.F. Van Hulst, M. Sauer (2004): Multistep energy transfer in single molecular photonic wires, J. Am. Chem. Soc. 126(21), 6514-6515.


D.-P. Herten, A. Biebricher, M. Heilemann, T. Heinlein, C. Müller, P. Schlüter, P. Tinnefeld, K.D. Weston, M. Sauer, J. Wolfrum (2004): Optical single molecule techniques for analytical and biological applications, Rec. Res. Devel. Phys. Chem. 7, 345-368

invited article


T.D.M. Bell, S. Habuchi, S. Masuo, I. Österling, K. Müllen, P. Tinnefeld, M. Sauer, M. van der Auweraer, J. Hofkens, F.C. De Schryver (2004): Single photon emission from a dendrimer containing eight perylene diimide chromophores, Aust. J. Chem. 57(12), 1169-1173.


P. Tinnefeld, V. Buschmann, K.D. Weston, A. Biebricher, D.-P. Herten, O. Piestert, T. Heinlein, M. Heilemann, M. Sauer (2004): How single-molecule photophysical studies complement ensemble methods for a better understanding of chromophores and interchromophore interactions, Rec. Res. Devel. Phys. Chem. 7, 95-125

invited article


H. Neuweiler, D.-P. Herten, N. Marme, J.-P. Knemeyer, O. Priestert, P. Tinnefeld, M. Sauer (2004): Probing conformational dynamics by photoinduced electron transfer, Proc SPIE 5322, 8-19.



M. Heilemann, V. Buschmann, O. Priestert, P. Tinnefeld, K.D. Weston, M. Sauer (2003): Development of a molecular photonic wire by means of multiparameter single molecule spectroscopy, Proc SPIE 4962, 38-46.

J. Hofkens, M. Cotlet, T. Vosch, P. Tinnefeld, K.D. Weston, C. Ego, A. Grimsdale, K. Müllen, D. Beljonne, J.L. Bredas, S. Jordens, G. Schweitzer, M. Sauer, F. De Schryver (2003): Revealing competitive Förster-type resonance energy-transfer pathways in single bichromophoric molecules, Proc. Natl. Acad. Sci. 100(23), 13146-13151.


T. Heinlein, M. Heilemann, D.-P. Herten, C. Müller, P. Tinnefeld, K.D. Weston, M. Sauer (2003): Spectrally-resolved fluorescence lifetime imaging microscopy (SFLIM) and coincidence analysis: new tools to study the organization of biomolecular machines, Proc SPIE 4962, 47-57.


T. Vosch, M. Cotlet, J. Hofkens, K. van der Biest, M. Lor, K.D. Weston, P. Tinnefeld, M. Sauer, L. Latterini, K. Müllen, F.C. De Schryver (2003): Probing Förster Type Energy Pathways in a first generation rigid dendrimer bearing two perylene imide chromophores, J. Phys. Chem. A 107(36), 6920-6931.


P. Tinnefeld, V. Buschmann, K.D. Weston, M. Sauer (2003): Direct Observation of collective blinking and energy transfer in a bichromophoric system, J. Phys. Chem. A 107(3), 323-327.



P. Tinnefeld, K.D. Weston, T. Vosch, M. Cotlet, T. Weil, J. Hofkens, K. Müllen, F.C. De Schryver, M. Sauer (2002): Antibunching in the emission of a single tetrachromophoric dendritic system, J. Am. Chem. Soc. 124(48), 14310-14311.

K.D. Weston, M. Dyck, P. Tinnefeld, C. Müller, D.-P. Herten, M. Sauer (2002): Measuring the number of independent emitters in single-molecule fluorescence images and trajectories using coincident photons, Anal. Chem. 74(20), 5342-5349.


M. Heilemann, D.-P. Herten, R. Heintzmann, C. Cremer, C. Müller, P. Tinnefeld, K.D. Weston, J. Wolfrum, M. Sauer (2002): High-resolution colocalization of single dye molecules by fluorescence lifetime imaging microscopy, Anal. Chem. 74(14), 3511-3517.



P. Tinnefeld, D.-P. Herten, M. Sauer (2001): Photophysical dynamics of single molecules studied by spectrally-resolved fluorescence lifetime-imaging microscopy (FLIM), J. Phys. Chem. A 105(34), 7989-8003.

P. Tinnefeld, C. Müller, M. Sauer (2001): Time-varying photon probability distribution of individual molecules at room temperature, Chem. Phys. Lett. 345(3-4), 252-258.



D.-P. Herten, P. Tinnefeld, M. Sauer (2000): Identification of single fluorescently labelled mononucleotide molecules in solution by spectrally resolved time-correlated single-photon counting, Appl. Phys. B 71, 765-771.

P. Tinnefeld, V. Buschmann, D.-P. Herten, K.-T. Han, M. Sauer (2000): Confocal fluorescence lifetime imaging microscopy (FLIM) at the single molecule level, Single Mol. 1(3), 215-223.


H. Irngartinger, P.W. Fettel, T. Escher, P. Tinnefeld, S. Nord, M. Sauer (2000): Substituent effects on redox properties and photoinduced electron transfer in isoxazolo-fullerenes, Eur. J .Org. Chem. 3, 455-465.