| Office | 089/2180-77547 (Frau Steger) |
| Telephone | 089/2180-77537 |
| Room | E1.003 |
| fiona.cole”at”cup.uni-muenchen.de |
Publications
Jonas Zähringer; Fiona Cole; Johann Bohlen; Florian Steiner; Izabela Kamińska; Philip Tinnefeld
Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy Journal Article
In: Light Sci Appl, vol. 12, no. 1, pp. 70, 2023, ISSN: 2047-7538.
@article{pmid36898993,
title = {Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy},
author = {Jonas Zähringer and Fiona Cole and Johann Bohlen and Florian Steiner and Izabela Kamińska and Philip Tinnefeld},
doi = {10.1038/s41377-023-01111-8},
issn = {2047-7538},
year = {2023},
date = {2023-03-01},
urldate = {2023-03-01},
journal = {Light Sci Appl},
volume = {12},
number = {1},
pages = {70},
abstract = {3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging. Here, we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer (GET) and the single-molecule switching by DNA-PAINT. We demonstrate <2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm. In 3D DNA-PAINT measurements, structural features, i.e., individual docking strands at distances of 3 nm, are directly resolved on DNA origami structures. pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information. Furthermore, we introduce local PAINT (L-PAINT), in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters. L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging. Here, we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer (GET) and the single-molecule switching by DNA-PAINT. We demonstrate <2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm. In 3D DNA-PAINT measurements, structural features, i.e., individual docking strands at distances of 3 nm, are directly resolved on DNA origami structures. pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information. Furthermore, we introduce local PAINT (L-PAINT), in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters. L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.
Martina Pfeiffer; Kateryna Trofymchuk; Simona Ranallo; Francesco Ricci; Florian Steiner; Fiona Cole; Viktorija Glembockyte; Philip Tinnefeld
Single antibody detection in a DNA origami nanoantenna Journal Article
In: iScience, vol. 24, no. 9, pp. 103072, 2021.
@article{Pfeiffer2021,
title = {Single antibody detection in a DNA origami nanoantenna},
author = {Martina Pfeiffer and Kateryna Trofymchuk and Simona Ranallo and Francesco Ricci and Florian Steiner and Fiona Cole and Viktorija Glembockyte and Philip Tinnefeld},
doi = {10.1016/j.isci.2021.103072},
year = {2021},
date = {2021-09-01},
urldate = {2021-09-01},
journal = {iScience},
volume = {24},
number = {9},
pages = {103072},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Luciano A. Masullo; Florian Steiner; Jonas Zähringer; Lucía F. Lopez; Johann Bohlen; Lars Richter; Fiona Cole; Philip Tinnefeld; Fernando D. Stefani
Pulsed Interleaved MINFLUX Journal Article
In: Nano Letters, vol. 21, no. 1, pp. 840–846, 2020.
@article{Masullo2020,
title = {Pulsed Interleaved MINFLUX},
author = {Luciano A. Masullo and Florian Steiner and Jonas Zähringer and Lucía F. Lopez and Johann Bohlen and Lars Richter and Fiona Cole and Philip Tinnefeld and Fernando D. Stefani},
doi = {10.1021/acs.nanolett.0c04600},
year = {2020},
date = {2020-12-01},
journal = {Nano Letters},
volume = {21},
number = {1},
pages = {840--846},
publisher = {American Chemical Society (ACS)},
keywords = {},
pubstate = {published},
tppubtype = {article}
}