Group of Prof. Tinnefeld - Faculty for Chemistry and Pharmacy

Research

Single-molecule sensing with DNA origami: The unique modularity and positioning ability of the DNA origami method provides exciting opportunities for the development of biosensors and molecular diagnostic tools. In our lab, we combine the tools of DNA nanotechnology and our expertise in single-molecule fluorescence techniques for the development of a range of different single-molecule sensors. More
Single-molecule biophysics: Protein and protein complexes are fascinating nanomachines performing key functions in living organisms. Improved over billion years of natural selection, protein complexes are fine-tuned by evolution to perform their function with unmatchable efficiency, and complex physicochemical mechanism are exploited by nature to control and modify their activity. Single-molecule fluorescence techniques allow us to directly look at these molecules while performing their activity, and therefore, to extract information about the conformational changes occurring during their inner working, or in response to external factors. More
Superresolution microscopy: Already since the early days of single-molecule localization microscopy (SMLM), our lab is involved in the development and application of related techniques, that enable a ~10-fold resolution enhancement compared to conventional fluorescence microscopy. Our most prominent examples are the inventions of direct stochastic optical reconstruction microscopy (dSTORM) in cooperation with the Sauer group (Würzburg University), and DNA-PAINT (points accumulation for imaging in nanoscale topography) together with the Simmel group (TU Munich). More
Single-molecule spectroscopy: Our lab performs optical spectroscopy at the highest level of sensitivity. The applied techniques allow us to detect single photons emitted from individual molecules. Depending on the underlying project, these photons are resolved spectrally, with respect to their polarization or temporal – usually in the picosecond to the second time domain. The latter method gives insight into fluorescence lifetime (the time a molecule spends in its excited state) or photon correlations (e.g. dynamics of single molecule emission or photon anti-bunching). More