Dr. Viktorija Glembockytė

@article{Grabenhorst2024,

title = {Engineering modular and tunablesingle-molecule sensors by decouplingsensing from signal output},

author = {Lennart Grabenhorst and Martina Pfeiffer and Thea Schinkel and Mirjam Kümmerlin and Gereon A. Brüggenthies and Jasmin B. Maglic and Florian Selbach and Alexander T. Murr and Philip Tinnefeld and Viktorija Glembockyte },

doi = {https://doi.org/10.1038/s41565-024-01804-0},

issn = {1748-3395},

year  = {2024},

date = {2024-11-07},

journal = {Nature Nanotechnology},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Full Site-Specific Addressability in DNA Origami-Templated Silica Nanostructures},

author = {Lea M. Wassermann and Michael Scheckenbach and Anna V. Baptist and Viktorija Glembockyte and Amelie Heuer-Jungemann},

url = {https://onlinelibrary.wiley.com/doi/10.1002/adma.202212024},

doi = {10.1002/adma.202212024},

year  = {2023},

date = {2023-04-25},

urldate = {2023-04-25},

journal = {Advanced Materials},

abstract = {DNA nanotechnology allows for the fabrication of nanometer-sized objects with high precision and selective addressability as a result of the programmable hybridization of complementary DNA strands. Such structures can template the formation of other materials, including metals and complex silica nanostructures, where the silica shell simultaneously acts to protect the DNA from external detrimental factors. However, the formation of silica nanostructures with site-specific addressability has thus far not been explored. Here, it is shown that silica nanostructures templated by DNA origami remain addressable for post silicification modification with guest molecules even if the silica shell measures several nm in thickness. The conjugation of fluorescently labeled oligonucleotides is used to different silicified DNA origami structures carrying a complementary ssDNA handle as well as DNA-PAINT super-resolution imaging to show that ssDNA handles remain unsilicified and thus ensure retained addressability. It is also demonstrated that not only handles, but also ssDNA scaffold segments within a DNA origami nanostructure remain accessible, allowing for the formation of dynamic silica nanostructures. Finally, the power of this approach is demonstrated by forming 3D DNA origami crystals from silicified monomers. These results thus present a fully site-specifically addressable silica nanostructure with complete control over size and shape.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid36594816,

title = {Gold Nanorod DNA Origami Antennas for 3 Orders of Magnitude Fluorescence Enhancement in NIR},

author = {Kateryna Trofymchuk and Karol Kołątaj and Viktorija Glembockyte and Fangjia Zhu and Guillermo P Acuna and Tim Liedl and Philip Tinnefeld},

doi = {10.1021/acsnano.2c09577},

issn = {1936-086X},

year  = {2023},

date = {2023-01-01},

urldate = {2023-01-01},

journal = {ACS Nano},

abstract = {DNA origami has taken a leading position in organizing materials at the nanoscale for various applications such as manipulation of light by exploiting plasmonic nanoparticles. We here present the arrangement of gold nanorods in a plasmonic nanoantenna dimer enabling up to 1600-fold fluorescence enhancement of a conventional near-infrared (NIR) dye positioned at the plasmonic hotspot between the nanorods. Transmission electron microscopy, dark-field spectroscopy, and fluorescence analysis together with numerical simulations give us insights on the heterogeneity of the observed enhancement values. The size of our hotspot region is ∼12 nm, granted by using the recently introduced design of NAnoantenna with Cleared HotSpot (NACHOS), which provides enough space for placing of tailored bioassays. Additionally, the possibility to synthesize nanoantennas in solution might allow for production upscaling.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Close2022,

title = {Maximizing the Accessibility in DNA Origami Nanoantenna Plasmonic Hotspots},

author = {Cindy Close and Kateryna Trofymchuk and Lennart Grabenhorst and Birka Lalkens and Viktorija Glembockyte and Philip Tinnefeld},

url = {https://doi.org/10.1002/admi.202200255},

doi = {10.1002/admi.202200255},

year  = {2022},

date = {2022-07-01},

urldate = {2022-07-01},

journal = {Advanced Materials Interfaces},

volume = {9},

issue = {24},

pages = {2200255},

publisher = {Wiley},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Pfeiffer2021,

title = {Single antibody detection in a DNA origami nanoantenna},

author = {Martina Pfeiffer and Kateryna Trofymchuk and Simona Ranallo and Francesco Ricci and Florian Steiner and Fiona Cole and Viktorija Glembockyte and Philip Tinnefeld},

doi = {10.1016/j.isci.2021.103072},

year  = {2021},

date = {2021-09-01},

urldate = {2021-09-01},

journal = {iScience},

volume = {24},

number = {9},

pages = {103072},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Glembockyte2021,

title = {DNA Origami Nanoantennas for Fluorescence Enhancement},

author = {Viktorija Glembockyte and Lennart Grabenhorst and Kateryna Trofymchuk and Philip Tinnefeld},

doi = {10.1021/acs.accounts.1c00307},

year  = {2021},

date = {2021-08-01},

urldate = {2021-08-01},

journal = {Acc Chem Res},

volume = {54},

number = {17},

pages = {3338--3348},

publisher = {American Chemical Society (ACS)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Trofymchuk2021,

title = {Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope},

author = {Kateryna Trofymchuk and Viktorija Glembockyte and Lennart Grabenhorst and Florian Steiner and Carolin Vietz and Cindy Close and Martina Pfeiffer and Lars Richter and Max L. Schütte and Florian Selbach and Renukka Yaadav and Jonas Zähringer and Qingshan Wei and Aydogan Ozcan and Birka Lalkens and Guillermo P. Acuna and Philip Tinnefeld},

doi = {10.1038/s41467-021-21238-9},

year  = {2021},

date = {2021-02-01},

urldate = {2021-02-01},

journal = {Nature Communications},

volume = {12},

number = {1},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34606673,

title = {Targetable Conformationally Restricted Cyanines Enable Photon-Count-Limited Applications*},

author = {Patrick Eiring and Ryan McLaughlin and Siddharth S Matikonda and Zhongying Han and Lennart Grabenhorst and Dominic A Helmerich and Mara Meub and Gerti Beliu and Michael Luciano and Venu Bandi and Niels Zijlstra and Zhen-Dan Shi and Sergey G Tarasov and Rolf Swenson and Philip Tinnefeld and Viktorija Glembockyte and Thorben Cordes and Markus Sauer and Martin J Schnermann},

doi = {10.1002/anie.202109749},

issn = {1521-3773},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Angew Chem Int Ed Engl},

volume = {60},

number = {51},

pages = {26685--26693},

abstract = {Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications, but their photon output can be limited by trans-to-cis photoisomerization. We recently demonstrated that appending a ring system to the pentamethine cyanine ring system improves the quantum yield and extends the fluorescence lifetime. Here, we report an optimized synthesis of persulfonated variants that enable efficient labeling of nucleic acids and proteins. We demonstrate that a bifunctional sulfonated tertiary amide significantly improves the optical properties of the resulting bioconjugates. These new conformationally restricted cyanines are compared to the parent cyanine derivatives in a range of contexts. These include their use in the plasmonic hotspot of a DNA-nanoantenna, in single-molecule Förster-resonance energy transfer (FRET) applications, far-red fluorescence-lifetime imaging microscopy (FLIM), and single-molecule localization microscopy (SMLM). These efforts define contexts in which eliminating cyanine isomerization provides meaningful benefits to imaging performance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Scheckenbach2021,

title = {Self-Regeneration and Self-Healing in DNA Origami Nanostructures},

author = {Michael Scheckenbach and Tom Schubert and Carsten Forthmann and Viktorija Glembockyte and Philip Tinnefeld},

doi = {10.1002/anie.202012986},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Angewandte Chemie International Edition},

volume = {60},

number = {9},

pages = {4931--4938},

publisher = {Wiley},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Isselstein2020,

title = {Self-Healing Dyes—Keeping the Promise?},

author = {Michael Isselstein and Lei Zhang and Viktorija Glembockyte and Oliver Brix and Gonzalo Cosa and Philip Tinnefeld and Thorben Cordes},

doi = {10.1021/acs.jpclett.9b03833},

year  = {2020},

date = {2020-05-01},

journal = {The Journal of Physical Chemistry Letters},

volume = {11},

number = {11},

pages = {4462--4480},

publisher = {American Chemical Society (ACS)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matikonda2020,

title = {Impact of Cyanine Conformational Restraint in the Near-Infrared Range},

author = {Siddharth S. Matikonda and Gabrielle Hammersley and Nikita Kumari and Lennart Grabenhorst and Viktorija Glembockyte and Philip Tinnefeld and Joseph Ivanic and Marcia Levitus and Martin J. Schnermann},

doi = {10.1021/acs.joc.0c00236},

year  = {2020},

date = {2020-04-01},

journal = {The Journal of Organic Chemistry},

volume = {85},

number = {9},

pages = {5907--5915},

publisher = {American Chemical Society (ACS)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Grabenhorst2020,

title = {Fluorophore photostability and saturation in the hotspot of DNA origami nanoantennas},

author = {Lennart Grabenhorst and Kateryna Trofymchuk and Florian Steiner and Viktorija Glembockyte and Philip Tinnefeld},

doi = {10.1088/2050-6120/ab6ac8},

year  = {2020},

date = {2020-02-01},

journal = {Methods and Applications in Fluorescence},

volume = {8},

number = {2},

pages = {024003},

publisher = {IOP Publishing},

keywords = {},

pubstate = {published},

tppubtype = {article}

}