Jonas Zähringer

@article{pmid36898993,

title = {Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy},

author = {Jonas Zähringer and Fiona Cole and Johann Bohlen and Florian Steiner and Izabela Kamińska and Philip Tinnefeld},

doi = {10.1038/s41377-023-01111-8},

issn = {2047-7538},

year  = {2023},

date = {2023-03-01},

urldate = {2023-03-01},

journal = {Light Sci Appl},

volume = {12},

number = {1},

pages = {70},

abstract = {3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging. Here, we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer (GET) and the single-molecule switching by DNA-PAINT. We demonstrate <2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm. In 3D DNA-PAINT measurements, structural features, i.e., individual docking strands at distances of 3 nm, are directly resolved on DNA origami structures. pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information. Furthermore, we introduce local PAINT (L-PAINT), in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters. L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kaminska2021,

title = {Graphene Energy Transfer for Single-Molecule Biophysics, Biosensing, and Super-Resolution Microscopy},

author = {Izabela Kamińska and Johann Bohlen and Renukka Yaadav and Patrick Schüler and Mario Raab and Tim Schröder and Jonas Zähringer and Karolina Zielonka and Stefan Krause and Philip Tinnefeld},

doi = {10.1002/adma.202101099},

year  = {2021},

date = {2021-05-01},

urldate = {2021-05-01},

journal = {Advanced Materials},

volume = {33},

number = {24},

pages = {2101099},

publisher = {Wiley},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Trofymchuk2021,

title = {Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope},

author = {Kateryna Trofymchuk and Viktorija Glembockyte and Lennart Grabenhorst and Florian Steiner and Carolin Vietz and Cindy Close and Martina Pfeiffer and Lars Richter and Max L. Schütte and Florian Selbach and Renukka Yaadav and Jonas Zähringer and Qingshan Wei and Aydogan Ozcan and Birka Lalkens and Guillermo P. Acuna and Philip Tinnefeld},

doi = {10.1038/s41467-021-21238-9},

year  = {2021},

date = {2021-02-01},

urldate = {2021-02-01},

journal = {Nature Communications},

volume = {12},

number = {1},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Masullo2020,

title = {Pulsed Interleaved MINFLUX},

author = {Luciano A. Masullo and Florian Steiner and Jonas Zähringer and Lucía F. Lopez and Johann Bohlen and Lars Richter and Fiona Cole and Philip Tinnefeld and Fernando D. Stefani},

doi = {10.1021/acs.nanolett.0c04600},

year  = {2020},

date = {2020-12-01},

journal = {Nano Letters},

volume = {21},

number = {1},

pages = {840--846},

publisher = {American Chemical Society (ACS)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Scheckenbach2020,

title = {DNA origami nanorulers and emerging reference structures},

author = {Michael Scheckenbach and Julian Bauer and Jonas Zähringer and Florian Selbach and Philip Tinnefeld},

doi = {10.1063/5.0022885},

year  = {2020},

date = {2020-11-01},

journal = {APL Materials},

volume = {8},

number = {11},

pages = {110902},

publisher = {AIP Publishing},

keywords = {},

pubstate = {published},

tppubtype = {article}

}