| Office | 089/2180-77547 (Frau Steger) |
| Telephone | 089 / 2180-77541 |
| Room | E1.027 |
| julian.bauer “at” cup.uni-muenchen.de |
Publications
Kristina Hübner; Mario Raab; Johann Bohlen; Julian Bauer; Philip Tinnefeld
Salt-Induced Conformational Switching of a Flat Rectangular DNA Origami Structure Journal Article
In: Nanoscale, vol. 14, pp. 7898 - 7905, 2022.
@article{nokey,
title = {Salt-Induced Conformational Switching of a Flat Rectangular DNA Origami Structure},
author = {Kristina Hübner and Mario Raab and Johann Bohlen and Julian Bauer and Philip Tinnefeld },
url = {https://doi.org/10.1039/D1NR07793G},
doi = {10.1039/D1NR07793G},
year = {2022},
date = {2022-05-11},
urldate = {2022-05-11},
journal = {Nanoscale},
volume = {14},
pages = { 7898 - 7905},
abstract = {A rectangular DNA origami structure is one of the most studied and often used motif for
applications in DNA nanotechnology. Here, we present two assays to study structural
changes in DNA nanostructures and reveal a reversible rolling-up of the rectangular DNA
origami structure induced by bivalent cations such as magnesium or calcium. First, we
applied one-color and two-color superresolution DNA-PAINT with protruding strands
along the long edges of the DNA origami rectangle. At increasing salt concentration, a
single line instead of two lines is observed as a first indicator of rolling-up. Two-color
measurements also revealed different conformations with parallel and angled edges.
Second, we placed a gold nanoparticle and a dye molecule at different positions on the
DNA origami structure. Distance dependent fluorescence quenching by the nanoparticle
reports on dynamic transitions as well as it provides evidence that the rolling-up occurs
preferentially along the diagonal of the DNA origami rectangle. The results will be helpful
to test DNA structural models and the assays presented will be useful to study further
structural transitions in DNA nanotechnology. },
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A rectangular DNA origami structure is one of the most studied and often used motif for
applications in DNA nanotechnology. Here, we present two assays to study structural
changes in DNA nanostructures and reveal a reversible rolling-up of the rectangular DNA
origami structure induced by bivalent cations such as magnesium or calcium. First, we
applied one-color and two-color superresolution DNA-PAINT with protruding strands
along the long edges of the DNA origami rectangle. At increasing salt concentration, a
single line instead of two lines is observed as a first indicator of rolling-up. Two-color
measurements also revealed different conformations with parallel and angled edges.
Second, we placed a gold nanoparticle and a dye molecule at different positions on the
DNA origami structure. Distance dependent fluorescence quenching by the nanoparticle
reports on dynamic transitions as well as it provides evidence that the rolling-up occurs
preferentially along the diagonal of the DNA origami rectangle. The results will be helpful
to test DNA structural models and the assays presented will be useful to study further
structural transitions in DNA nanotechnology.
applications in DNA nanotechnology. Here, we present two assays to study structural
changes in DNA nanostructures and reveal a reversible rolling-up of the rectangular DNA
origami structure induced by bivalent cations such as magnesium or calcium. First, we
applied one-color and two-color superresolution DNA-PAINT with protruding strands
along the long edges of the DNA origami rectangle. At increasing salt concentration, a
single line instead of two lines is observed as a first indicator of rolling-up. Two-color
measurements also revealed different conformations with parallel and angled edges.
Second, we placed a gold nanoparticle and a dye molecule at different positions on the
DNA origami structure. Distance dependent fluorescence quenching by the nanoparticle
reports on dynamic transitions as well as it provides evidence that the rolling-up occurs
preferentially along the diagonal of the DNA origami rectangle. The results will be helpful
to test DNA structural models and the assays presented will be useful to study further
structural transitions in DNA nanotechnology.
Michael Scheckenbach; Julian Bauer; Jonas Zähringer; Florian Selbach; Philip Tinnefeld
DNA origami nanorulers and emerging reference structures Journal Article
In: APL Materials, vol. 8, no. 11, pp. 110902, 2020.
@article{Scheckenbach2020,
title = {DNA origami nanorulers and emerging reference structures},
author = {Michael Scheckenbach and Julian Bauer and Jonas Zähringer and Florian Selbach and Philip Tinnefeld},
doi = {10.1063/5.0022885},
year = {2020},
date = {2020-11-01},
journal = {APL Materials},
volume = {8},
number = {11},
pages = {110902},
publisher = {AIP Publishing},
keywords = {},
pubstate = {published},
tppubtype = {article}
}