Michael Scheckenbach

@article{nokey,

title = {Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging},

author = {Michael Scheckenbach and Cindy Close and Julian Bauer and Lennart Grabenhorst and Fiona Cole and Jens Köhler and Siddharth S. Matikonda and Lei Zhang and Thorben Cordes and Martin J. Schnermann and Andreas Herrmann and Philip Tinnefeld and Alan M. Szalai and Viktorija Glembockyte},

doi = {https://doi.org/10.1101/2025.01.08.631860},

year  = {2025},

date = {2025-01-10},

journal = {bioRxiv preprint},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Monitoring the Coating of Single DNA Origami Nanostructures with a Molecular Fluorescence Lifetime Sensor},

author = {Michael Scheckenbach and Gereon Andreas Brüggenthies and Tim Schröder and Karina Betuker and Lea Wassermann and Philip Tinnefeld and Amelie Heuer-Jungemann and Viktorija Glembockyte},

url = {https://doi.org/10.1101/2024.10.28.620667},

doi = {10.1101/2024.10.28.620667},

year  = {2024},

date = {2024-10-31},

urldate = {2024-10-31},

journal = {bioRxiv preprint},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bringing Attomolar Detection to the Point-of-Care with Nanopatterned DNA Origami Nanoantennas},

author = {Renukka Yaadav and Kateryna Trofymchuk and Mihir Dass and Vivien Behrendt and Benedikt Hauer and Jan Schütz and Cindy Close and Michael Scheckenbach and Giovanni Ferrari and Leoni Maeurer and Sophia Sebina and Viktorija Glembockyte and Tim Liedl and Philip Tinnefeld},

url = {https://doi.org/10.1101/2024.10.14.618183},

doi = {10.1101/2024.10.14.618183},

year  = {2024},

date = {2024-10-17},

urldate = {2024-10-17},

journal = {bioRxiv preprint},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Full Site-Specific Addressability in DNA Origami-Templated Silica Nanostructures},

author = {Lea M. Wassermann and Michael Scheckenbach and Anna V. Baptist and Viktorija Glembockyte and Amelie Heuer-Jungemann},

url = {https://onlinelibrary.wiley.com/doi/10.1002/adma.202212024},

doi = {10.1002/adma.202212024},

year  = {2023},

date = {2023-04-25},

urldate = {2023-04-25},

journal = {Advanced Materials},

abstract = {DNA nanotechnology allows for the fabrication of nanometer-sized objects with high precision and selective addressability as a result of the programmable hybridization of complementary DNA strands. Such structures can template the formation of other materials, including metals and complex silica nanostructures, where the silica shell simultaneously acts to protect the DNA from external detrimental factors. However, the formation of silica nanostructures with site-specific addressability has thus far not been explored. Here, it is shown that silica nanostructures templated by DNA origami remain addressable for post silicification modification with guest molecules even if the silica shell measures several nm in thickness. The conjugation of fluorescently labeled oligonucleotides is used to different silicified DNA origami structures carrying a complementary ssDNA handle as well as DNA-PAINT super-resolution imaging to show that ssDNA handles remain unsilicified and thus ensure retained addressability. It is also demonstrated that not only handles, but also ssDNA scaffold segments within a DNA origami nanostructure remain accessible, allowing for the formation of dynamic silica nanostructures. Finally, the power of this approach is demonstrated by forming 3D DNA origami crystals from silicified monomers. These results thus present a fully site-specifically addressable silica nanostructure with complete control over size and shape.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid36735241,

title = {DNA Origami Curvature Sensors for Nanoparticle and Vesicle Size Determination with Single-Molecule FRET Readout},

author = {Ece Büber and Tim Schröder and Michael Scheckenbach and Mihir Dass and Henri G Franquelim and Philip Tinnefeld},

doi = {10.1021/acsnano.2c11981},

issn = {1936-086X},

year  = {2023},

date = {2023-02-01},

urldate = {2023-02-01},

journal = {ACS Nano},

abstract = {Particle size is an important characteristic of materials with a direct effect on their physicochemical features. Besides nanoparticles, particle size and surface curvature are particularly important in the world of lipids and cellular membranes as the cell membrane undergoes conformational changes in many biological processes which leads to diverging local curvature values. On account of that, it is important to develop cost-effective, rapid and sufficiently precise systems that can measure the surface curvature on the nanoscale that can be translated to size for spherical particles. As an alternative approach for particle characterization, we present flexible DNA nanodevices that can adapt to the curvature of the structure they are bound to. The curvature sensors use Fluorescence Resonance Energy Transfer (FRET) as the transduction mechanism on the single-molecule level. The curvature sensors consist of segmented DNA origami structures connected via flexible DNA linkers incorporating a FRET pair. The activity of the sensors was first demonstrated with defined binding to different DNA origami geometries used as templates. Then the DNA origami curvature sensors were applied to measure spherical silica beads having different size, and subsequently on lipid vesicles. With the designed sensors, we could reliably distinguish different sized nanoparticles within a size range of 50-300 nm as well as the bending angle range of 50-180°. This study helps with the development of more advanced modular-curvature sensing devices that are capable of determining the sizes of nanoparticles and biological complexes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Scheckenbach2021,

title = {Self-Regeneration and Self-Healing in DNA Origami Nanostructures},

author = {Michael Scheckenbach and Tom Schubert and Carsten Forthmann and Viktorija Glembockyte and Philip Tinnefeld},

doi = {10.1002/anie.202012986},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Angewandte Chemie International Edition},

volume = {60},

number = {9},

pages = {4931--4938},

publisher = {Wiley},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Scheckenbach2020,

title = {DNA origami nanorulers and emerging reference structures},

author = {Michael Scheckenbach and Julian Bauer and Jonas Zähringer and Florian Selbach and Philip Tinnefeld},

doi = {10.1063/5.0022885},

year  = {2020},

date = {2020-11-01},

journal = {APL Materials},

volume = {8},

number = {11},

pages = {110902},

publisher = {AIP Publishing},

keywords = {},

pubstate = {published},

tppubtype = {article}

}