Dr. Andrés Vera Gómez

@article{pmid34904657,

title = {A simple and general approach to generate photoactivatable DNA processing enzymes},

author = {Merve-Zeynep Kesici and Philip Tinnefeld and Andrés Manuel Vera},

doi = {10.1093/nar/gkab1212},

issn = {1362-4962},

year  = {2021},

date = {2021-12-01},

urldate = {2021-12-01},

journal = {Nucleic Acids Res},

abstract = {DNA processing enzymes, such as DNA polymerases and endonucleases, have found many applications in biotechnology, molecular diagnostics, and synthetic biology, among others. The development of enzymes with controllable activity, such as hot-start or light-activatable versions, has boosted their applications and improved the sensitivity and specificity of the existing ones. However, current approaches to produce controllable enzymes are experimentally demanding to develop and case-specific. Here, we introduce a simple and general method to design light-start DNA processing enzymes. In order to prove its versatility, we applied our method to three DNA polymerases commonly used in biotechnology, including the Phi29 (mesophilic), Taq, and Pfu polymerases, and one restriction enzyme. Light-start enzymes showed suppressed polymerase, exonuclease, and endonuclease activity until they were re-activated by an UV pulse. Finally, we applied our enzymes to common molecular biology assays and showed comparable performance to commercial hot-start enzymes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vera2021,

title = {Cohesin-dockerin code in cellulosomal dual binding modes and its allosteric regulation by proline isomerization},

author = {Andrés Manuel Vera and Albert Galera-Prat and Michał Wojciechowski and Bartosz Różycki and Douglas V. Laurents and Mariano Carrión-Vázquez and Marek Cieplak and Philip Tinnefeld},

doi = {10.1016/j.str.2021.01.006},

year  = {2021},

date = {2021-06-01},

urldate = {2021-06-01},

journal = {Structure},

volume = {29},

number = {6},

pages = {587--597.e8},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vera2020,

title = {Single-Molecule Approved Surface Passivation},

author = {Andrés Manuel Vera and Philip Tinnefeld},

doi = {10.1016/j.str.2020.11.009},

year  = {2020},

date = {2020-12-01},

journal = {Structure},

volume = {28},

number = {12},

pages = {1269--1270},

publisher = {Elsevier BV},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Liu2020,

title = {High force catch bond mechanism of bacterial adhesion in the human gut},

author = {Zhaowei Liu and Haipei Liu and Andrés M. Vera and Rafael C. Bernardi and Philip Tinnefeld and Michael A. Nash},

doi = {10.1038/s41467-020-18063-x},

year  = {2020},

date = {2020-08-01},

journal = {Nature Communications},

volume = {11},

number = {1},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kramm2020,

title = {DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability},

author = {Kevin Kramm and Tim Schröder and Jerome Gouge and Andrés Manuel Vera and Kapil Gupta and Florian B. Heiss and Tim Liedl and Christoph Engel and Imre Berger and Alessandro Vannini and Philip Tinnefeld and Dina Grohmann},

doi = {10.1038/s41467-020-16702-x},

year  = {2020},

date = {2020-06-01},

journal = {Nature Communications},

volume = {11},

number = {1},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hellenkamp2018,

title = {Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study},

author = {Björn Hellenkamp and Sonja Schmid and Olga Doroshenko and Oleg Opanasyuk and Ralf Kühnemuth and Soheila Rezaei Adariani and Benjamin Ambrose and Mikayel Aznauryan and Anders Barth and Victoria Birkedal and Mark E. Bowen and Hongtao Chen and Thorben Cordes and Tobias Eilert and Carel Fijen and Christian Gebhardt and Markus Götz and Giorgos Gouridis and Enrico Gratton and Taekjip Ha and Pengyu Hao and Christian A. Hanke and Andreas Hartmann and Jelle Hendrix and Lasse L. Hildebrandt and Verena Hirschfeld and Johannes Hohlbein and Boyang Hua and Christian G. Hübner and Eleni Kallis and Achillefs N. Kapanidis and Jae-Yeol Kim and Georg Krainer and Don C. Lamb and Nam Ki Lee and Edward A. Lemke and Brié Levesque and Marcia Levitus and James J. McCann and Nikolaus Naredi-Rainer and Daniel Nettels and Thuy Ngo and Ruoyi Qiu and Nicole C. Robb and Carlheinz Röcker and Hugo Sanabria and Michael Schlierf and Tim Schröder and Benjamin Schuler and Henning Seidel and Lisa Streit and Johann Thurn and Philip Tinnefeld and Swati Tyagi and Niels Vandenberk and Andrés Manuel Vera and Keith R. Weninger and Bettina Wünsch and Inna S. Yanez-Orozco and Jens Michaelis and Claus A. M. Seidel and Timothy D. Craggs and Thorsten Hugel},

doi = {10.1038/s41592-018-0085-0},

year  = {2018},

date = {2018-08-01},

journal = {Nature Methods},

volume = {15},

number = {9},

pages = {669--676},

publisher = {Springer Science and Business Media LLC},

keywords = {},

pubstate = {published},

tppubtype = {article}

}